Contramuffin@lemmy.world
on 02 Jul 22:38
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Explanation: CRISPR-Cas9 is most commonly known to be a gene editor, but since its initial discovery, people have found that with some minor tweaks, CRISPR can be made to do a bunch of other things, things that biologists could have only dreamed of previously.
If you introduce a mutation that deactivates the Cas9 protein, the entire CRISPR complex still binds to DNA. Then, you can essentially glue whatever you want onto this modified Cas9 to produce a bunch of different effects:
deactivated Cas9, no additional modifications: blocks other proteins from touching the DNA, which suppresses a gene without destroying it
glue on some transcriptional activators: makes the cell express a gene
glue on some fluorescent proteins: makes your DNA glow, lets you see where it is
ChillCapybara@discuss.tchncs.de
on 03 Jul 02:27
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Well done! I zoomed in and felt dumb. But thanks to your succinct explanation, I now have a sweet Dunning-Kruger high
BootyEnthusiast@lemmy.dbzer0.com
on 02 Jul 22:53
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I want to >!fuck!< CRISPRi
DeltaWingDragon@sh.itjust.works
on 02 Jul 23:01
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Your spoiler failed
riskable@programming.dev
on 03 Jul 01:22
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Sadly there are two spoiler types i know of :/
for me the spoiler works because i use the “second one?”
call_me_xale@lemmy.zip
on 02 Jul 23:32
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“Did you know…”
general_kitten@sopuli.xyz
on 03 Jul 05:00
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“…that in terms of male human and female CRISPR-Cas9 variant reproduction, CRISPRi is the most compatible CRISPR-Cas9 variant for humans? Not only are they in the genetic perturbation group which is mostly comprised of various methods, CRISPRi are an average of…”
ZILtoid1991@lemmy.world
on 03 Jul 05:08
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I prefer meowscarada.
joenforcer@midwest.social
on 04 Jul 06:23
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It’s a drawing! It’s fiction! And if you come with the “normalizing” argument, then your “Y’all need Jesus” is normalizing the christofascism that will destroy this world.
joenforcer@midwest.social
on 04 Jul 13:40
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Y’all need therapy. Mostly for taking a joke waaaaaaay too seriously.
I dunno. For hundreds of thousands of years we’ve been selectively breeding puppies.
Puppies
general_kitten@sopuli.xyz
on 03 Jul 04:45
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In a way one could argue genetic engineering is one of the oldest sciences ever.
And it started with dogs, or maybe agriculture, depends on what one’s definition of science is.
emergencyfood@sh.itjust.works
on 06 Jul 04:37
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Whatever we did to pugs is definitely not cute. Or okay.
threaded - newest
Explanation: CRISPR-Cas9 is most commonly known to be a gene editor, but since its initial discovery, people have found that with some minor tweaks, CRISPR can be made to do a bunch of other things, things that biologists could have only dreamed of previously.
If you introduce a mutation that deactivates the Cas9 protein, the entire CRISPR complex still binds to DNA. Then, you can essentially glue whatever you want onto this modified Cas9 to produce a bunch of different effects:
Well done! I zoomed in and felt dumb. But thanks to your succinct explanation, I now have a sweet Dunning-Kruger high
I want to >!fuck!< CRISPRi
Your spoiler failed
FTFY đź‘Ť
Sadly there are two spoiler types i know of :/
for me the spoiler works because i use the “second one?”
“Did you know…”
“…that in terms of male human and female CRISPR-Cas9 variant reproduction, CRISPRi is the most compatible CRISPR-Cas9 variant for humans? Not only are they in the genetic perturbation group which is mostly comprised of various methods, CRISPRi are an average of…”
I prefer meowscarada.
Y’all need Jesus. And an assigned FBI agent.
It’s a drawing! It’s fiction! And if you come with the “normalizing” argument, then your “Y’all need Jesus” is normalizing the christofascism that will destroy this world.
Y’all need therapy. Mostly for taking a joke waaaaaaay too seriously.
Can’t help it, it’s most compatible
Genetic engineering has never been this cute
I dunno. For hundreds of thousands of years we’ve been selectively breeding puppies.
Puppies
In a way one could argue genetic engineering is one of the oldest sciences ever. And it started with dogs, or maybe agriculture, depends on what one’s definition of science is.
Whatever we did to pugs is definitely not cute. Or okay.