Contramuffin@lemmy.world
on 02 Jul 22:38
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Explanation: CRISPR-Cas9 is most commonly known to be a gene editor, but since its initial discovery, people have found that with some minor tweaks, CRISPR can be made to do a bunch of other things, things that biologists could have only dreamed of previously.
If you introduce a mutation that deactivates the Cas9 protein, the entire CRISPR complex still binds to DNA. Then, you can essentially glue whatever you want onto this modified Cas9 to produce a bunch of different effects:
deactivated Cas9, no additional modifications: blocks other proteins from touching the DNA, which suppresses a gene without destroying it
glue on some transcriptional activators: makes the cell express a gene
glue on some fluorescent proteins: makes your DNA glow, lets you see where it is
ChillCapybara@discuss.tchncs.de
on 03 Jul 02:27
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Well done! I zoomed in and felt dumb. But thanks to your succinct explanation, I now have a sweet Dunning-Kruger high
BootyEnthusiast@lemmy.dbzer0.com
on 02 Jul 22:53
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I want to >!fuck!< CRISPRi
DeltaWingDragon@sh.itjust.works
on 02 Jul 23:01
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Your spoiler failed
riskable@programming.dev
on 03 Jul 01:22
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Sadly there are two spoiler types i know of :/
for me the spoiler works because i use the “second one?”
call_me_xale@lemmy.zip
on 02 Jul 23:32
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“Did you know…”
general_kitten@sopuli.xyz
on 03 Jul 05:00
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“…that in terms of male human and female CRISPR-Cas9 variant reproduction, CRISPRi is the most compatible CRISPR-Cas9 variant for humans? Not only are they in the genetic perturbation group which is mostly comprised of various methods, CRISPRi are an average of…”
ZILtoid1991@lemmy.world
on 03 Jul 05:08
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I prefer meowscarada.
joenforcer@midwest.social
on 04 Jul 06:23
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It’s a drawing! It’s fiction! And if you come with the “normalizing” argument, then your “Y’all need Jesus” is normalizing the christofascism that will destroy this world.
joenforcer@midwest.social
on 04 Jul 13:40
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Y’all need therapy. Mostly for taking a joke waaaaaaay too seriously.
I dunno. For hundreds of thousands of years we’ve been selectively breeding puppies.
Puppies
general_kitten@sopuli.xyz
on 03 Jul 04:45
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In a way one could argue genetic engineering is one of the oldest sciences ever.
And it started with dogs, or maybe agriculture, depends on what one’s definition of science is.
threaded - newest
Explanation: CRISPR-Cas9 is most commonly known to be a gene editor, but since its initial discovery, people have found that with some minor tweaks, CRISPR can be made to do a bunch of other things, things that biologists could have only dreamed of previously.
If you introduce a mutation that deactivates the Cas9 protein, the entire CRISPR complex still binds to DNA. Then, you can essentially glue whatever you want onto this modified Cas9 to produce a bunch of different effects:
Well done! I zoomed in and felt dumb. But thanks to your succinct explanation, I now have a sweet Dunning-Kruger high
I want to >!fuck!< CRISPRi
Your spoiler failed
FTFY đź‘Ť
Sadly there are two spoiler types i know of :/
for me the spoiler works because i use the “second one?”
“Did you know…”
“…that in terms of male human and female CRISPR-Cas9 variant reproduction, CRISPRi is the most compatible CRISPR-Cas9 variant for humans? Not only are they in the genetic perturbation group which is mostly comprised of various methods, CRISPRi are an average of…”
I prefer meowscarada.
Y’all need Jesus. And an assigned FBI agent.
It’s a drawing! It’s fiction! And if you come with the “normalizing” argument, then your “Y’all need Jesus” is normalizing the christofascism that will destroy this world.
Y’all need therapy. Mostly for taking a joke waaaaaaay too seriously.
Can’t help it, it’s most compatible
Genetic engineering has never been this cute
I dunno. For hundreds of thousands of years we’ve been selectively breeding puppies.
Puppies
In a way one could argue genetic engineering is one of the oldest sciences ever. And it started with dogs, or maybe agriculture, depends on what one’s definition of science is.